Today's topic for #whywednesday is why does this phuture doctor care to enhance #multiplexing#proteomics. Recall from my #mondaymotivation post that it takes 2 hours to run one sample through the liquid chromatography-mass spectrometer. As of now, up to 22 to peptide samples can be analyzed at once as there are two ways to label the peptides and eleven types of tags that can be added.
When scientists collaborate with clinics to research the cause of a disease, scientists are often handed over large batches of patient's tissue samples at a time to analyze. If scientists are dealing with large batches of samples at a time and each sample takes up to 2 hours to run (not including sample prep time), it makes sense as to why multiplexing was developed in the first place and why there are efforts to continue to enhance this workflow. #getshitdone and #getoutoflabataresonabletime#gradlife#phdlife .
Here is the general workflow after labeling, tagging, and combing the peptides into one sample. The sample is first injected into a high-performance liquid chromatography (HPLC) instrument where the peptides are separated due to their polarity interaction with the solvent flowing through the HPLC column. Once separated, they pass through electrospray ionization before reaching the mass spectrometer (MS). This technique uses high voltage to evaporate the solvent, leaving a stream of charged peptide ions ready to be injected into the MS. The MS then bombards the peptide ions with electrons causing the peptides to fragment into smaller charged ions.
With MS, we can determine the mass of the peptide based on their mass-to-charge ratio, identifying the peptide, and correlate it to the protein it came from. Here's what is so cool about multiplexing. Because the 22 different peptide samples from the patients were labeled and tagged, the scientist can tell which peptide pertained to which patient! Post statistical analysis compares and quantifies the abundance of proteins present in patients with and without the #disease. Ultimately leading to identifying if the proteins in the #brain, #heart, or #liver may be the cause of #Alzheimer's disease.
God loves it when on paper, it looks as if the odds are stacked against you. I was reminded of this, this week when I was reading the story of when Gideon and the Israelites defeated the Midianites. The Midianites had an army of 135,000 men while the Israelites had an army of only 32,000 men. Despite being outmanned more than 4 men to 1, God told Gideon he had too many warriors and that with their current numbers, when the Israelites won, they would boast that they won by their own might. He thus downsized the Israelite army to only 300 men. This left the Israelite army outnumbered 450 men to 1! However, with God’s help, they demolished the Midianite army. God will place us in situations that leave us without a doubt that it was only by His help that we achieved the victory. When your MCAT score, your GPA, your Step scores etc. etc. etc. don’t seem like enough to get you to where you want to be, remember, God loves it that way because when you look back at how you made it anyway, you will remember you didn’t do it on your own. Stay encouraged because God loves a good underdog story just like the rest of us.
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Didn’t catch the #BacktoSchool Live session on #MinecraftEdu’s new underwater features & #STEM lessons? Watch it on-demand by following the link in our bio!
Stuck this on my #siggraph badge today. Real-time rendering of a volumetric texture. I recently updated the housing design, now there's a power switch. I need to shoot another video but enjoy this old repost! #3dprinted#fusion360
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Have you taken your daughter to the Robot Exhibit at OMSI yet? It’s there until September 3rd so check it out! Play tic-tac-toe against a robot or try out one of the many other hands-on robots 🤖
Meet Sri! Sri is majoring in Maths @macquarieuni and is in the final year of her Education degree. Her career aspiration is to be a high school Mathematics teacher; “I’m very lucky that two of my passions (Maths and Teaching) combine so well together.”
And we have no doubt she’ll make great teacher! .
When asked why she chose to study Maths MQ Sri says “I chose Maths because it was something I enjoyed at school and was always aware of its importance in Science. I’m studying at MQ because it offered the degree that I wished to do, as well as a bunch of other units/subjects that weren’t available at other universities. I also fell in love with the campus when I visited during Open Day during my HSC!” .
Sri is also the President of the Mathematics Society at MQ! “Being the President has presented many challenges such as managing a team and meeting deadlines whilst managing Uni study and work, but has also been a rewarding experience when people come along to our events and recognise our hard work. My team works so well together and are all driven and passionate about Mathematics, which makes it so much more of an enjoyable experience.” .
When she’s not busy well, with everything, you can find Sri playing with and training her recently adopted pup or reading a good novel. .
Sri will be on campus @Macquarie_Uni#OpenDay on Aug 18 with the rest of the #Maths and #Stats team at the Science Hub. She’ll also be at the Career Speed Dating Booth (12-1pm) to answer all your study, student, career & MQ related questions so don’t be shy and come say hi! 👋 Openday.mq.edu.au/.
RepostBy @jpmajor: "Tupan Patera, a 50-mile-wide volcanic crater on Jupiter's moon Io imaged by NASA's #Galileo spacecraft on Oct. 15, 2001. Re: the colors, according to @NASA: "The black material is recent, still-warm lava. The yellow is presumed to be a mix of sulfurous compounds, and the green appears to form where red sulfur has interacted with the dark lavas." The sheer walls surrounding the edge of the crater are 3,000 feet—over half a mile—high....this one crater could contain most if not all of my home state of RI. #space#stem#science#nasa#space#solarsystem#science#astronomy"
You know when it all gets a bit much and you've got a bunch of edits to do to a paper before submission, sometimes it's just best to get away somewhere quite like a splendidly relaxing coffee shop 😊. That's how I've been spending this #workwednesday 😁 thanks @ridemybikecafe
Author Katie Slivensky is on YAYOMG! today sharing her inspirations behind bringing the Seismic Seven to life, interesting facts about supervolcanoes, and her advice for kids who love science! ✨⠀
💥Link in @yayomgofficial bio for the interview!⠀
📚SYNOPSIS: This action-packed, science-infused adventure from the critically acclaimed author of The Countdown Conspiracy follows a group of seven kids racing to save the world from a deadly supervolcano. Perfect for fans of Chris Grabenstein and Peter Lerangis.⠀
Brianna Dobson has been chosen to work with world-renowned geologist Dr. Grier in Yellowstone National Park for the summer, and she couldn’t be more excited!⠀
But then Dr. Grier tells her and the other kids on the project the real reason she’s invited them to Yellowstone: A massive supervolcano in the park is about to erupt—and if they can’t stop it, Earth will be plunged into an endless winter, and civilization will be destroyed.⠀
Bri and her new friends are ready and willing to help stop the eruption, but unexpected dangers threaten to foil their efforts. Will the Seismic Seven be able to defy nature and save the world?⠀
I have more wedding flowers coming off the 3D printer all the time! ⚘ So much so that it's time to order some more 3D printer filament. Just polished off my latest kilogram of glow in the dark material for my various wedding projects. All the bridesmaid bouquets are coming together!
I'm recording this whole process, so I can't wait to share a video of all these wedding projects when I'm done.
Get your ducks in a row before the weekend! It’s time to celebrate all things Science, Technology, Engineering & Maths at the Townsville STEM Faire, CityLibraries Thuringowa 9am - 2:30pm. #science#scienceweek#townsville#nq#stem
You know what is more attractive than the latest fashions? .
People who SEE and ACCEPT others for who they are = Attractive .
Compassion = Attractive .
Confidence = Attractive .
Judgmental = Not attractive .
Self absorbed = Not attractive
Double tap if you agree
A little STEM and team building all in one! I’ve been doing this several years and I think I first read it about it on @teachingwithamountainview’s blog! 💙 Their task is to design and build a tool that can stack the cups, using only string and a rubber band. It’s the perfect amount of challenge and when they figure out the perfect combo, they are so proud! It’s a great beginning of the year activity!
Meet Dr Cathy Foley: Newly appointed Chief Scientist here at your national science agency. You might say she's a superconductor for all things research and accolades.
"Cathy is a world-renowned physicist and science leader most noted for her work developing superconducting systems including a technology called LANDTEM which uses superconductors to create three-dimensional maps of underground ore bodies.
And in her latest venture, Cathy has just been appointed as our Chief Scientist. This is one of the most senior roles in the organisation and she says her priority will be putting science, STEM and women in science back in the spotlight." Check out the link in our bio to read Cathy's story.